Cell bio lab report Essay

Purpose: During this experiment we compared the hemagglutination reaction of control Con A solution at 2 mg/ml in Con A buffer with the hemagglutination reaction of your own purified Con A sample that you diluted previously at 2 mg/ml in Con A buffer. The purpose of this lab was to determine the strength of the reaction by performing serial dilutions on both the Con A sample and the control Con A sample, and determine through observations whether or not addition of galactose or mannose will inhibit this reaction. I hypothesize that the Con A + galactose solutions will have partial agglutination and partial no agglutination, and the Con A + mannose solutions will have all no agglutination. Results: Rows A and B had half agglutination and half no agglutination, while row C had all no agglutination. Row D had half agglutination and half partial agglutination, while row E had 4 columns with agglutination and 8 columns with no agglutination. Row F had complete agglutination throughout. Con A reaction plate (Row/Column) Reaction A1-A6 (Control) Agglutination/inhibited A7-A12 (Control) No Agglutination/not inhibited B1-B6 (Con A + galactose) Agglutination/inhibited B7-B12 (Con A + galactose) No Agglutination/not inhibited C1-C12 (Con A + mannose) No Agglutination/not inhibited D1-D5 (Sample) Agglutination/inhibited D6-D12 (Sample) Partial agglutination/inhibited E1-E4 (Con A + galactose) Agglutination/inhibited E5-E12 (Con A + galactose) No Agglutination/not inhibited F1-F12 (Con A + mannose) No agglutination/not inhibited G1-G12 ((-)Control) Partial agglutination/inhibited H1-H12 (RBCs) Partial agglutination/inhibited Discussion: My hypothesis was proven correct, the Con A + galactose solutions did have partial agglutination and partial no agglutination, and the Con A + mannose solutions did have all no agglutination. This means that in the Con A + galactose solutions the ones that had agglutination the red cells aggregated with the lectin and sediment in the form of a uniform layer that covered the whole bottom of the well including the slopes, and in the Con A + mannose solutions the ones that failed to have agglutination take place covered only part of the bottom. We found through this experiment that the control Con A sample and the Con A sample had very similar strengths of reaction. Conclusion: In this experiment through serial dilutions on a 96 well plate we determined the strength of the each reaction on both the Con A sample and the control sample. We found through our experiment that the addition of galactose or mannose will partially inhibit or inhibit the reaction between our Con A sample and control Con A sample.